Coding

Part:BBa_K3513000:Design

Designed by: Huandi Xu   Group: iGEM20_NFLS   (2020-10-23)


endoglucanase with signal peptide and linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 106
    Illegal NotI site found at 1250
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1163
    Illegal BamHI site found at 299
    Illegal XhoI site found at 661
    Illegal XhoI site found at 910
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 441
    Illegal NgoMIV site found at 1366
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 345


Design Notes

Add signal peptide to make it secreted out of the cells

Source

Origin source: Streptomyces Coelicolor. Then was engineered by the team.

References